ANTI-HUMAN CD253/TRAIL (2E5) COLORWHEEL DYE-READY MAB

General description

ColorWheelColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheelColorWheel^® antibody with any ColorWheelColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheelColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheelColorWheel^® technology requires both ColorWheelColorWheel^® antibody and ColorWheelColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone 2E5 is a mouse monoclonal antibody that detects CD253 (TRAIL). It targets an epitope within the extracellular domain.

Immunogen

A recombinant fragment corresponding to 187 amino acids from the extracellular domain of human CD253 (TRAIL).

Application

Quality Control Testing

Evaluated by Flow Cytometry in CD253-transfected HEK293 cells.

Flow Cytometry Analysis: Staining of one million CD253-transfected HEK293 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1122, Anti-Human CD253/TRAIL (2E5) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million CD253-transfected HEK293 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1122, Anti-Human CD253/TRAIL (2E5) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Tumor necrosis factor ligand superfamily member 10 (UniProt: P50591; also known as Apo-2 ligand, Apo-2L, TNF-related apoptosis-inducing ligand, Protein TRAIL, CD253) is encoded by the TNFSF10 (also known as APO2L, TRAIL) gene (Gene ID: 8743) in human. TRAIL/CD253 is a single-pass type II membrane protein with a cytoplasmic domain (aa 1-17), a transmembrane domain (aa 18-38), and an extracellular domain (aa 19-281). It is widely expressed and is most predominant in the spleen, lung, and prostate tissue. In addition to the membrane form, a soluble form has also been reported. It exists as a homotrimer where one unit of TRAIL (TNFSF10) interacts with three TRAIL1 (TNFSF10A) or TRAIL2 (TNFSF10B) monomers. TRAIL is a cytokine that binds to TRAIL receptors. Its activity can be modulated by binding to decoy receptors TRAILR3 and TRAILR4. TNFSF10 gene is reported to be a p53-transcriptional gene with two p53 binding sites. Its silencing by siRNA is reported to suppress caspase 3 and caspase 7 activities even after treatment with the DNA damaging agents, such as Adriamycin. TRAIL/CD253 protein expression is shown to be elevated in Adriamycin-treated breast cancer cells. However, it is also shown to engage the apoptosis of tumor cells in a p53 independent manner. Soluble recombinant TRAIL has been shown to induce apoptosis in a wide variety of cancer cells in vitro and to specifically limit tumor growth without damaging normal cells and tissues in vivo. (Ref.: Kuribayashi, K., et al. (2008). Cancer Biol. Ther. 7(12); 2034-2038; Kim, MH., et al. (2004). Biochem. Biophys. Res. Commun. 321(4); 930-935).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.