General description
ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
Specificity
Clone CB38 is a mouse monoclonal antibody that detects human platelet glycoprotein 4 (CD36).
Immunogen
Human myeloid cells.
Application
Quality Control Testing:
Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).
Flow Cytometry Analysis: Staining of one million Human peripheral blood mononuclear cells (PBMC) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1025, Anti-CD36 Antibody (CB38) ColorWheel® Mouse mAb and Cat. No. CWD-PE ColorWheel® activated Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgM isotype control.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Compatibility
Requires ColorWheel® Dye (Sold Separately)
Target description
Platelet glycoprotein 4 (UniProt: P16671; also known as Fatty acid translocase, FAT, Glycoprotein IIIb, GPIIIB, Leukocyte differentiation antigen CD36, PAS IV, PAS-4, Platelet collagen receptor, Platelet glycoprotein IV, GPIV, Thrombospondin receptor, CD36) is encoded by the CD36 (also known as GP3B, GP4) gene (Gene ID: 948) in human. CD36 is a multi-pass membrane glycoprotein that contains two cytoplasmic domains (aa 2-7 and 462-472), one extracellular domain (aa 30-439), and two transmembrane domains (aa 8-29 and 440-461). It acts as a receptor for a broad range of ligands that can be of proteinaceous nature like thrombospondin, fibronectin, collagen, and amyloid-beta or of lipidic nature, such as oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids and bacterial diacylated lipopeptides. They are generally multivalent and can engage multiple receptors simultaneously. The resulting formation of CD36 clusters initiates signal transduction and internalization of receptor-ligand complexes. Cellular responses to these ligands are involved in angiogenesis, inflammatory response, fatty acid metabolism, and dietary fat processing in the intestine. It binds long-chain fatty acids and facilitates their transport into cells. In taste receptor cells, CD36 is reported to mediate an increase in intracellular calcium levels by long-chain fatty acids, leading to the activation of the gustatory neurons. Upon binding of oxLDL or amyloid peptide 42, it interacts with the TLR4:TLR6 heterodimer and the complex is then internalized and triggers inflammatory response, leading to NF-kB-dependent production of several cytokines. Mutations in CD36 gene are associated with Platelet glycoprotein IV deficiency that is characterized by macrothrombocytopenia.
Physical form
Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.
Reconstitution
1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.
Legal Information
ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
- UPC:
- 51202410
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- CWA-1025-25UL