ANTI-HUMAN CD49A/ITGA1 (TS2/7) COLORWHEEL DYE-READY MAB

General description

ColorWheelColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheelColorWheel^® antibody with any ColorWheelColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheelColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheelColorWheel^® technology requires both ColorWheelColorWheel^® antibody and ColorWheelColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone TS2/7 is a mouse monoclonal antibody that detects Integrin- a1 (CD49a).

Immunogen

Human CTL line directed against HLA-DR.

Application

Quality Control Testing

Evaluated by Flow Cytometry in HeLa cells.

Flow Cytometry Analysis: Staining of one million HeLa cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1114, Anti-Human CD55 (143-30) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million HeLa cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1119, Anti-Human CD49a/ITGA1 (TS2/7) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Integrin alpha-1 (UniProt: P56199; also known as CD49 antigen-like family member A, Laminin and collagen receptor, VLA-1, CD49a) is encoded by the ITGA1 gene (Gene ID: 3672) in human. CD49a/ITGA1 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-28), which is subsequently cleaved off to generate the mature form that contains a large extracellular domain (aa 29-1141), transmembrane domain (aa 1142-1164), and a short cytoplasmic tail (aa 1165-1179). It contains an Integrin I domain (VWFA; aa 161-360) and integrins with I-domain do not undergo proteolytic cleavage. CD49a/ITGA1 forms a heterodimer with integrin 1 that serves as a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence GFPGER in collagen. CD49a/ITGA1 is shown to be frequently up-regulated in pancreatic ductal adenocarcinoma (PDAC) and associated precursor lesions. It is required for collagen-induced PDAC cell attachment and spreading. Expression of ITGA1-specific collagens within the pancreatic cancer microenvironment significantly correlate with poor patient prognosis. CD49a/ITGA1 levels are also reported to be frequently up-regulated in other cancers, such as melanoma, prostate, bladder, liver. and myeloma. Cd49a/ITGA1 is also required for TGF-β/collagen-induced EMT and metastasis. (Ref.: Gharibi, A., et al. (2017). Sci. Rep. 7; 10060).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.