ANTI-HUMAN CD69 (FN50) COLORWHEEL DYE-READY MAB

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone FN50 is a mouse monoclonal antibody that detects human CD69.

Immunogen

Anti-μ stimulated human B lymphocytes.

Application

Quality Control Testing:

Evaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC) stimulated with PMA/Ionomycin.

Staining of one million human peripheral blood mononuclear cells (PBMC) stimulated with Ionomycin/PMA (1 μg/mL, 4 h) was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1037, Anti-Human CD69 (FN50) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of PE-conjugated Mouse IgG1 isotype control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Early activation antigen CD69 (UniProt: Q07108; Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69) is encoded by the CD69 (also known as CLEC2C) gene (Gene ID: 969) in human. CD69 is a single-pass type II membrane disulfide-linked homodimeric protein with a cytoplasmic domain (aa 1-40), a transmembrane domain (aa 41-61), and an extracellular domain (aa 62-199). It is a C-type lectin and a member of the natural killer (NK) receptor family. It is an early activation marker that is expressed in hematopoietic stem cells, activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhans cells, and platelets. It is induced by antigens, mitogens, or activators of PKC on the surface of T and B-lymphocytes and by interaction of IL-2 with the p75 IL-2R on the surface of NK cells. CD69 is involved in immune cell homeostasis and regulates T cell-mediated immune response through the control of Th17 cell differentiation. Expression of CD69 by activated T lymphocytes is reported to trigger an anti-inflammatory mechanism mediated by Galectin-1, which regulates the immune response and prevents pathogenic Th17 responses. CD69 knockout mice are shown to develop an exacerbated form of collagen-induced arthritis. (Ref.: de la Fuente, H., et al. (2014). Mol. Cell. Biol. 34(13); 2479-2487; Ziegler SF et al., (1994). Stem Cells. 12(5); 456-65).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.