ANTI-HUMAN CD71 (RVS-10) COLORWHEEL DYE-READY MAB

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone RVS-10 is a mouse monoclonal antibody that detects human Transferrin receptor protein 1 (CD71).

Immunogen

Full-length human Transferrin receptor protein 1 (CD71).

Application

Quality Control Testing:

Evaluated by Flow Cytometry in K562 cells.

Flow Cytometry Analysis: Staining of one million human K562 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1039, Anti-Human CD71 (RVS-10) ColorWheel^® Dye-Ready mAb and Cat. No. CWD-PE ColorWheel^® activated Phycoerythrin (PE) Dye or an equivalent amount of Mouse IgG1 Isotype Control.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Transferrin receptor protein 1 (UniProt: P02786; also known as TR, TfR, TfR1, Trfr, T9, p90, CD71) is encoded by the TFRC gene (Gene ID: 7037) in human. Iron is essential for multiple metabolic processes and is incorporated into many proteins in the form of cofactors such as heme and iron-sulfur clusters. CD71 (Transferrin receptor 1) facilitates the uptake of iron at the cell surface by internalizing diferric transferrin. It is a single-pass type II membrane protein that contains a cytoplasmic domain (aa 1-67), a transmembrane domain (aa 68-88), and a long extracellular domain (aa 89-760). Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes where acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. The transferrin/CD71 system is shown to be important for erythroblasts and lymphocytes, T and B cells and its deficiency leads to anemia and compromised immune responses. Mutations in TFRC gene have been linked to immunodeficiency 46 that is characterized by early-onset chronic diarrhea, recurrent infections, intermittent neutropenia, and thrombocytopenia. (Ref.: Jennifer, B., et al. (2020). Commun. Biol. 3; Article 621; Wang, S., et al. (2020). Haematologica 105(8); 2071-2082).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL of PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.