ANTI-HUMAN CD90/THY-1 (5E10) COLORWHEEL DYE-READY MAB

General description

ColorWheelColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheelColorWheel^® antibody with any ColorWheelColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheelColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheelColorWheel^® technology requires both ColorWheelColorWheel^® antibody and ColorWheelColorWheel^® dye for Flow Cytometry application.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone 5E10 is a mouse monoclonal antibody that detects Thy-1 membrane glycoprotein (CD90).

Immunogen

HEL human erythroleukemia cells.

Application

Quality Control Testing

Evaluated by Flow Cytometry in Jurkat cells.

Flow Cytometry Analysis: Staining of one million Jurkat cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1063, Anti-Human CD90/Thy-1 (5E10) ColorWheel Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Staining of one million Jurkat cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1063, Anti-Human CD90/Thy-1 (5E10) ColorWheel Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Thy-1 membrane glycoprotein (UniProt: P04216; also known as CDw90, Thy-1 antigen, CD90) is encoded by the THY1 gene (Gene ID: 7070) in human. Thy-1 is a highly conserved, GPI-anchored membrane glycoprotein with one Ig V-type superfamily domain (aa 20-126). It is synthesized with a signal peptide (aa 1-19) and a propeptide (aa 131-161) that are subsequently cleaved off to generate the mature form (aa 20-130). It lacks an intracellular domain but is located in the outer leaflet of lipid rafts at the cell plasma membrane allowing signaling functions by cis- and trans-interactions with G inhibitory proteins, the Src and c-fyn kinases, and tubulin. Thy-1 is a major cell surface protein found on both T-cells and in the brain. It plays a key role in cell-cell and cell-ligand interactions during synaptogenesis and other events in the brain. It also plays a role in the regulation of axon growth and nerve regeneration, T cell activation and apoptosis, leukocytes and melanoma cell adhesion and migration, and fibroblast proliferation and migration in wound healing. Thy-1 mRNA and protein expression has been reported in several cancer types including liver, myeloid, skin, and brain. It is considered as a marker for glioblastoma stem cells. Reduced expression of Thy-1 is shown to enhance the expression of osteogenic and adipogenic differentiation of mesenchymal stem cells. (Ref.: Sauzay, C., et al. (2019). Front. Cell Dev. Biol. 7; 66; Moraes, DA., et al. (2016). Stem Cell Res. Ther. 7; Article 97).

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.