ANTI-HUMAN HISTONE H2A.X (1C22) COLORWHEEL DYE-READY MAB

General description

ColorWheel^® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel^® antibody with any ColorWheel^® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel^® antibody and dye is lyophilized for long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel^® technology requires both ColorWheel^® antibody and ColorWheel^® dye for Flow Cytometry application. This ColorWheel^® Dye-Ready monoclonal antibody was developed using our proprietary ZooMAb^® recombinant antibody technology. It offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

Specificity

Clone 1C22 is a rabbit monoclonal antibody that detects Histone H2AX. It targets an epitope within 15 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Histone H2AX.

Application

Quality Control Testing

Evaluated by Flow Cytometry in Fixed and permeabilized HepG2 cells.

Flow Cytometry Analysis: Intracellular staining of one million fixed and permeabilized HepG2 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1130, Anti-Human Histone H2A.X (1C22) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of Cat. No. CWA-1053 Rabbit IgG isotype control and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Tested Applications

Flow Cytometry Analysis: Intracellular staining of one million fixed and permeabilized Hep G2 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1130, Anti-Human Histone H2A.X (1C22) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye or the equivalent amount of Cat. No. CWA-1053 Rabbit IgG isotype control and Cat. No. CWDS-APC ColorWheelColorWheel^® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Flow Cytometry in Fixed and permeabilized HepG2 cells.

Flow Cytometry Analysis: Intracellular staining of one million fixed and permeabilized HepG2 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1130, Anti-Human Histone H2A.X (1C22) ColorWheelColorWheel^® Dye-Ready mAb and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye or the equivalent amount of Cat. No. CWA-1053 Rabbit IgG isotype control and Cat. No. CWDS-PE ColorWheelColorWheel^® Antibody-Ready Phycoerythrin (PE) Dye.

Compatibility

Requires ColorWheel^® Dye (Sold Separately)

Target description

Histone H2AX (UniProt: P16104; also known as H2a/x, Histone H2A.X) is encoded by the H2AX (also known as H2AFX) gene (gene ID: 3014) in human. Histones are highly conserved basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. They play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes, which limits DNA accessibility to the cellular machineries that require DNA as a template. The histone H2A.X is a variant member of the H2A family of histones that is distinguished from other H2A histones by a unique carboxy-terminal sequence. This unique sequence is highly conserved throughout eukaryotic evolution and is rapidly phosphorylated by ATM or ATR at serine 139 in mammals in response to DNA double-strand breaks. H2A.X phosphorylation is important in the formation of a stable repair complex at the site of DNA damage. Phosphorylation of H2A.X is important in the formation of a stable repair complex at the site of DNA damage. This phosphorylation is a very rapid response to DNA damage, occurring within as little as one minute after exposure to ionizing radiation. (Ref.: Paull, TT., et al. (2000). Curr. Biol. 10(15); 886-895; Rogakou, EP., et al. (1998). J. Biol. Chem. 273(10); 5858-5868).
This ColorWheel^® Dye-Ready monoclonal antibody was developed using our ZooMAb^® recombinant monoclonal antibody (Cat. No. ZRB1663). It offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

Reconstitution

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

Legal Information

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.