General description
IgA is the highest generated antibody isotype. IgAs are mainly secreted in the intestinal mucosa.
Anti-Human IgA (α-chain specific) antibody is specific for the α-chain of IgA in humans. The product reacts with human IgA when tested against purified human IgA, IgG, IgM, and Bence Jones κ and lambda myeloma proteins.
Immunoglobulin A (IgA) exists in two isotypic forms: IgA1 and IgA2. It is produced as secretory IgA (sIgA) and serum IgA. sIgA exists in a monomeric form with two heavy and two light chains. Serum IgA exists in the dimeric form linked by disulfide bind with an additional J chain. IgA is found in saliva, serum, colostrum and nasal fluid.
Specificity
The antibody preparation is specific for human IgA when tested against purified human IgA, IgG, IgM, Bence Jones kappa and lambda myeloma proteins.
Immunogen
IgA purified from normal human colostrum
Application
Anti-Human IgA (α-chain specific) antibody is suitable for use in:
- enzyme-linked immunosorbent assay (ELISA)
- quantitative precipitin assay (2.5-5 mg/mL)
- immunoelectrophoresis
- Ouchterlony double diffusion (1:16)
Biochem/physiol Actions
IgA is a secretory glycoprotein antibody that regulates the physiological balance between commensal intestinal bacteria and the defenses of the host immune system. It prevents the commensal microorganisms from invading the intestines.
Immunoglobulin A (IgA) mediates antibody-dependent cell-mediated cytotoxicity (ADCC) by binding to leukocytes and enhances microbial destruction. It acts as the first line of defense in antigen clearance. IgA initiates the alternative pathway of the complement system to perform protective functions. It reduces inflammation stimulated by the classical pathway complement activation. IgA prevents proteolysis and inhibits the uptake of bacterial, viral or antigen attachment.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing, or storage in "frost-free" freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 51201806
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- I9889-2ML
- Temperature Control Device:
- Yes
