Anti-Human IgG (Fc specific), F(ab')2 fragment antibody produced in goat

General description

IgG antibody plays a crucial role in humoral immune responses such as phagocytosis, complement activation, placental transport and cell surface-receptor binding. Anti-human IgG (Fc specific), F(ab′)₂ fragment antibody can be used as a capture antibody in antibody-binding assay for soluble ICAM-1-Fc. Goat anti-human-IgG (Fc specific) F(ab′)₂ antibody reacts specifically with human IgG but does not react with other immunoglobulins.

IgG is the main type of antibody in human serum, secreted by the B cells. It consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are linked by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4.

Immunogen

Purified human IgG

Application

Anti-Human IgG (Fc specific), F(ab′)₂ fragment antibody produced in goat has been used in studying the antibody binding assay of soluble intercellular adhesion molecule-1 (ICAM-1)-Fc.

Anti-human IgG (Fc specific), F(ab′)₂ fragment antibody can be used in ELISA to determine human IgG1-Fc protein.

Biochem/physiol Actions

IgG stimulates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a crucial role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG might be associated with allergy. It has the longest serum half life.

Other Notes

Antibody adsorbed with mouse and rat IgG

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% sodium azide

Preparation Note

Adsorbed to reduce background with mouse or rat samples.
Useful when trying to avoid background staining due to the presence of Fc receptors.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.