Anti-Human IgG (gamma-chain specific), F(ab')2 fragment-Peroxidase antibody produced in goat (C15-1314-965)

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders . Anti-Human IgG (γ-chain specific), (F(ab′)₂) fragment-Peroxidase antibody is specific for human IgG when tested against human IgA, IgG, IgM, Bence Jones κ, and λ myeloma proteins.

Immunogen

Purified human IgG

Application

Anti-Human IgG (γ-chain specific), (F(ab′)₂) fragment-Peroxidase antibody is suitable for use in ELISA and western blot .

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)

Peroxidase-conjugated goat anti-human IgG (Fab specific) antibody was used for western blot and ELISA analysis of yeast cultures genetically modified to produced recombinant Fab fragments. Anti-Human IgG antibody was used at a 1:10,000 dilution in PBS and incubated for 1 hour at room temperature. Binding in ELISA assays was detected with a O-phenylene- diamine substrate solution (Sigma).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative

Preparation Note

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: goat. Quality Level: 200. conjugate: peroxidase conjugate. antibody form: affinity isolated antibody. antibody product type: secondary antibodies. clone: polyclonal. form: buffered aqueous solution. species reactivity: human. technique(s): direct ELISA: 1:10,000, western blot: suitable. shipped in: dry ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Pictograms: GHS07,GHS09. Signal Word: Warning. Hazard Statements: H315 - H317 - H319 - H411. Precautionary Statements: P261 - P264 - P273 - P280 - P302 + P352 - P305 + P351 + P338. Hazard Classifications: Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.