ANTI-HUMAN IGG (WHOLE MOLECULE) PEROXIDASE CONJUGATE ANTIBODY DEVELOPED IN RABBIT IGG FRACTION OF ANTISERUM (C15-1315-494)

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders.

Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. IgG is usually found as a monomer. IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. About 70 percent of the total immunoglobulin consists of IgG.

Specificity

Rabbit Anti-Human IgG (whole molecule)-Peroxidase antibody binds to human IgG.

Immunogen

purified human IgG

Application

Anti-Human IgG (whole molecule)-Peroxidase antibody produced in rabbit has been used in:
enzyme-linked immunosorbent assay (ELISA)
western blotting
microarray analysis

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Enzyme-linked immunosorbent assay (1 paper)
Western Blotting (1 paper)

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT

Preparation Note

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 200. conjugate: peroxidase conjugate. antibody form: IgG fraction of antiserum. antibody product type: secondary antibodies. clone: polyclonal. form: buffered aqueous solution. species reactivity: human. technique(s): direct ELISA: 1:40,000, dot blot: 1:80,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150. shipped in: dry ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Pictograms: GHS08. Signal Word: Danger. Hazard Statements: H317 - H334. Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313. Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.