General description
HuR (Hu-antigen R, ELAV-like protein 1, ELAV1) is an RNA bindnig protein that contains 3 RNA-binding domains. It is known to binds AU-rich elements if mRNA. It regulates gene expression by destabilizing their mRNA. It is known to be upregulated in certain cancers such as breast and gastric cancers. Some of its targets include cFos, IL-3, and BRCA1 mRNAs. HuR has also been shown to be involved in the regulation of VEFG-A and NFkB expression in certain cell types. It activation and accumulation are also linked to many energy signaling molcules such as that of the PI3K/Akt pathway and AMPK.
Specificity
Detects HuR
Other species have not been tested.
Immunogen
Epitope: N-terminus
Synthetic peptide corresponding to the N-terminus of human HuR.
Application
Immunofluorescent ICC Analysis:
Research Category
Signaling
Research Sub Category
Insulin/Energy Signaling
RNA Binding Protein (RBP)
Use Anti-HuR Antibody (Rabbit Polyclonal Antibody) validated in WB, IF to detect HuR also known as ELAV-like 1, Hu antigen R.
Quality
Western Blot Analysis:
The antibody detected HuR at 1:500-1:1,000 dilutions in Jurkat cell lysate resolved via SDS-PAGE and transferred to PVDF.
Target description
36 kDa
Linkage
Replaces: AB5831
Physical form
Affinity purified polyclonal precipitated in ammonium sulfate.
Rabbit polyclonal precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Analysis Note
Control
Jurkat cell lysate
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 41106300
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- 07-1735
- Temperature Control Device:
- Yes
