Anti-ICAT Antibody (C15-1316-784)

General description

Beta-catenin-interacting protein 1 (UniProt Q9NSA3; also known as Beta-catenin-interacting protein ICAT, Inhibitor of beta-catenin and Tcf-4) is encoded by the CTNNBIP1 (also known as ICAT) gene (Gene ID 56998) in human. Inhibitor of beta-catenin and TCF (ICAT) negatively regulates T-cell factor/lymphoid enhancer factor (TCF/LEF)–beta-catenin transcriptional activity by competing against TCF/LEF for beta-catenin binding. ICAT gene knockout leads to premature death of newborn mice with gut, kidney, and craniofacial anomalies, implicating an important role of ICAT in epithelial establishment/maintenance and neural crest cell differentiation. The motility of melanoma cells through 3D matrices in vitro relies on two interconvertible modes of movements. ICAT is reported to suppress the expression of developmentally downregulated 9 (NEDD9) of Wnt/beta-catenin signaling pathway in human Lu1205 melanoma cells, resulting in a shift from the Rac-dependent mesenchymal movement to the Rho/ROCK-dependent amoeboid motility in vitro and enhanced colonization of transplanted Lu1205 in the lungs of nude mice in vivo.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human ICAT.

Application

Detect ICAT/CTNNBIP1 using this rabbit Polyclonal Anti-ICAT Antibody, Cat. No. ABC939, validated for use in Immunocytochemistry, Western Blotting and Immunoprecipitation.

Immunoprecipitation Analysis: A representative lot co-immunoprecitated beta-catenin with ICAT from lysates of five human melanoma cell lines (Lu1205, WM852, Dauv-1, Mel501, and Skmel3) (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).
Western Blotting Analysis: A representative lot detected ICAT in Lu1205, Mel501, human melanoma cells, and Skmel3 (Domingues, M.J., et al. (2014). Cancer Res. 74(7):1983-1995).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Immunocytochemistry in A431, HeLa, HUVEC and cells.

Immunocytochemistry Analysis: 4 µg/mL of this antibody detected ICAT in A431, HeLa, HUVEC cells.

Target description

~9 kDa calculated

Physical form

Protein G Purified

Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): Xenopus (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), zebrafish (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_064633. UniProt accession no.: Q9NSA3. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... CTNNBIP1(56998). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.