Anti-IGPR-1 Antibody (C15-1316-750)

General description

IGPR-1 or Immunoglobulin and proline-rich receptor 1, also called Transmembrane and immunoglobulin domain-containing protein 2 and encoded by the human gene TMIGD2/IGPR1 is a widely expressed epithelial and endothelial membrane protein that plays a role in cell-cell interactions, cell migration and angiogenesis. IGPR-1 regulates cellular morphology, homophilic cell aggregation, and cell-cell interaction. IGPR-1 activity also modulates actin stress fiber formation and focal adhesion and reduces cell migration. Silencing of expression of IGPR-1 by small interfering RNA (siRNA) and by ectopic overexpression in endothelial cells showed that IGPR-1 regulates capillary tube formation in vitro, and in B16F melanoma cells engineered to express IGPR-1 displayed extensive angiogenesis in the mouse Matrigel angiogenesis model. Moreover, IGPR-1, through its proline-rich cytoplasmic domain, associates with multiple Src homology 3 (SH3)-containing signaling proteins, including SH3 protein interacting with Nck (SPIN90/WISH), bullous pemphigoid antigen-1, and calcium channel β2.

Immunogen

Epitope: Cytoplasmic domain

Linear peptide corresponding to the cytoplasmic domain of the human mature form of IGPR-1.

Application

Anti-IGPR-1 antibody is an antibody against IGPR-1 for use in western blotting, ICC & IHC.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Western Blotting Analysis: 2 µg/mL from a representative lot detected IGPR-1 in 10 µg of HepG2 cell lysate, human placenta tissue lysate, HUVEC cell lysate, and L9 cell lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected IGPR-1 in IGPR-1 transfected PAE cell lysates (Rahimi, N., et al. (2012). Mol Cell Biol. 23(9):1646-1556.).

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected IGPR-1 in IGPR-1 transfected PAE cells (Rahimi, N., et al. (2012). Mol Cell Biol. 23(9):1646-1556.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected IGPR-1 in human colon cancer tissues (Rahimi, N., et al. (2012). Mol Cell Biol. 23(9):1646-1556.).

Quality

Evaluated by Western Blotting in PC-12 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IGPR-1 in 10 µg of PC-12 cell lysate.

Target description

~58 kDa observed. Uniprot describes two isoforms at 31 kDa and 30 kDa The pre-mature and mature form of this protein may be observed at 32 kDa and 58 kDa (Rahimi, N., et al. (2012). Mol Cell Biol. 23(9):1646-1556.). Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: polyclonal. species reactivity: rat, human. concentration: 1 . mg/mL. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. NCBI accession no.: NP_001162597. UniProt accession no.: Q96BF3. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... TMIGD2(126259). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.