ANTI-IKAROS ANTIBODY (C005B-240639)

General description

IKAROS, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. IKAROS has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.

Immunogen

KLH-conjugated linear peptide corresponding to human IKAROS.

Application

Anti-IKAROS Antibody detects level of IKAROS & has been published & validated for use in WB & IC.

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected IKAROS in ReNcell human neural progenitor cells.

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected IKAROS on 10 µg of HeLa cell lysate.

Target description

~65 kDa observed. (Martin-Ibanez, R., et al. (2010). The Journal of Comparative Neurology. 518:329–351.) An uncharacterized band appears at ~57 kDa in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: rat, human, mouse. technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_006051. UniProt accession no.: Q13422. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... IKZF1(10320)
mouse ... Ikzf1(22778)
rat ... Ikzf1(305501). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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