ANTI-IKKBETA ANTIBODY

General description

IKKbeta (IkB Kinase, IKKbeta, I-Kappa-B kinase-beta) is a member of the IKK complex which is composed of IKKalpha, IKKbeta, IKKgamma and IKAP. IkB Kinase (IKK) complex is a critical component in NFkB regulation. In a majority of unstimulated cells, the NFkB transcription factors exist in their inactive form and are retained in the cytoplasm by the bound inhibitory IkB proteins. For NFkB to become activated, it must first disassociate from the inhibitor IkB. This occurs via the serine phosphorylation of IkB on Ser32 and Ser36. Post receptor stimulation, most notably by TNFalpha, NIK, a serine/threonine kinase, becomes activated and phosphorylates IKKalpha and IKKbeta. Phosphorylation of 2 sites at the activation loop of IKK beta is essential for activation of IKK by TNF and IL1. Once activated, IKKbeta autophosphorylates, which in turn decreases IKK activity and prevents prolonged activation of the inflammatory response. Additionally, IKKbeta activity can also be regulated by MEKK-1. IKK then binds to and phosphorylates IkBalpha on the two serine residues. This phosphorylation marks IkB for ubiquination and destruction by the proteosome. This free the NFkB complex and allows it to translocates to the nucleus.

Specificity

This antibody recognizes IKKbeta, does not cross reacts with IKKα or IKKγ.

Immunogen

Rabbit anti-IKKβ polyclonal antibody was raised against a peptide corresponding to amino acids 662 to 680 of human IKKβ (3,4), which differs from the corresponding murine sequence by one amino acid.

Application

Detect IKKβ using this Anti-IKKβ Antibody validated for use in WB, IH(P).

Optimal working dilutions must be determined by end user.

Immunohistochemistry(paraffin):
Representative images from a previous lot.
Optimal Staining of IKKβ Polyclonal Antibody: HeLa Cells

Western blot: 1:500 to 1:1000 from a previous lot was tested on whole cell lysate from Jurkat cells can be used as positive control and an approximately 87 kDa band should be detected.

Research Category
Signaling

Research Sub Category
Immunological Signaling

Quality

Routinely evaluated by Western Blot on A431 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected IKK BETA on 10 μg of A431 lysates.

Target description

~87 kDa

Linkage

Replaces: AB16522

Physical form

Format: Purified

Purified

Purified rabbit polyclonal IgG in PBS containing 0.02% sodium azide.

Storage and Stability

Stable at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
A431 cell lysate, whole cell lysate from Jurkat cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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