Anti-IL-21 Antibody (C15-1317-280)

General description

Interleukin-21 (UniProt Q9HBE4; also known as IL-21, Za11) is encoded by the IL21 (also known as CVID11) gene (Gene ID 59067) in human. IL-21 is a type I four-alpha-helical bundle cytokine primarily produced by CD4+ T cells and NKT cells. The heterodimeric receptor IL-21R is dominantly expressed by B cells and shares the common cytokine receptor gamma chain (γc) with receptors for IL-2, IL-4, IL-7, IL-9, and IL-15. Studies indicate that IL-21 potently regulates both adaptive and innate immune responses and possesses widely pleiotropic functions. IL-21 has also been linked to autoimmune disorders, allergies, inflammatory diseases, as well as the pathogenesis of cancer. Human IL-21 is initially translated with an N-terminal 22 aa signal peptide sequence, which is later removed to produce the 133 aa mature IL-21.

Specificity

Expected to react with pro- and mature IL-21 of both isoform 1 and isoform 2 (IL-21iso).

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to human IL-21 sequence near the C-terminus.

Application

Flow Cytometry Analysis: 3.0 µg of this antibody detected IL-21 in human peripheral blood mononuclear cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This rabbit Polyclonal Anti-IL-21 Antibody, Cat. No. ABF184 is validated for use in Western Blotting, Flow Cytometry, for the detection of IL-21.

Quality

Evaluated by Western Blotting in human IL-21 transfected HEK293 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected IL-21 in 10 µg of human IL-21 transfected HEK293 cell lysate.

Target description

~17 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): bovine (based on 100% sequence homology), porcine (based on 100% sequence homology). technique(s): flow cytometry: suitable, western blot: suitable. NCBI accession no.: NP_068575. UniProt accession no.: Q9HBE4. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... IL21(59067). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.