Anti-IL-31 Antibody (C15-1317-883)

General description

Interleukin-31 (IL-31) is a T-cell cytokine that activates STAT3 and possibly STAT1 and STAT5 through the IL-31 heterodimeric receptor composed of IL31RA and OSMR. IL-31 is similar to IL-6 type cytokines and is produced mainly by T helper type 2 cells. The ligand-induced oligomerization of a dimeric receptor complex containing IL-31 receptor A and oncostatin M receptor is the mediator of IL-31 activity. These proteins activate the JAK/STAT and the AKT signaling pathways in response to IL-31 binding. RNA levels of IL-31 receptor A and oncostatin M receptor are induced in activated monocytes and expressed highly in epithelial cells. Overexpression of IL-31 in transgenic mice results in the development of pruritis, alopecia, and skin lesions. Mutations of IL-31 in humans may result in atopic dermatitis, suggesting that IL-31 may play a role in skin immunity and may represent a novel target for antipruritic drug development.

Specificity

This antibody recognizes the N-terminus of IL-31.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to the N-terminus of human IL-31.

Application

Anti-IL-31 Antibody is a highly specific rabbit polyclonal antibody, that targets IL-31 & has been tested in western blotting & IHC.

Immunohistochemistry Analysis: 10 µg/mL from a representative lot detected IL-31 in rat skeletal muscle tissue.

Research Category
Signaling

Research Sub Category
Developmental Signaling

Quality

Evaluated by Western Blotting in RAW264.7 cell lysate.

Western Blotting Analysis: 2.5 µg/mL of this antibody detected IL-31 in 15 µg of RAW264.7 cell lysate.

Target description

~30 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
RAW264.7 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat. species reactivity (predicted by homology): human (immunogen homology). technique(s): immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: Q6EAL8. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... IL31(386653). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.