Anti-IL-33 Antibody (C15-1317-258)

General description

IL-33 is a proinflammatory protein that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ST2 receptor, leading to the activation of MAP kinases and NF-kappaB. IL-33 induces production of helper T-cell type 2 (TH2) cytokines, including IL-4, IL-5 and IL-13, and is a known mediator of many inflammation responses. Cat. No. ABF108, Anti-IL-33, is an affinity purified rabbit polyclonal antibody that recognizes epitopes in the C-terminal half of murine IL-33. Reactivity to rat or other species has not been tested.

Immunogen

Epitope: C-Terminal

His-tagged recombinant protein corresponding to the C-Terminal of mouse IL-33.

Application

Anti-IL-33 Antibody is an antibody against IL-33 for use in Western Blotting, Flow Cytometry.

Flow Cytometry Analysis: 0.25 µg of this antibody from a representative lot detected IL-33 in 1X10E6 monensin treated MEF1 cells.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Quality

Evaluated by Western Blotting in MEF1 cell lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected IL-33 in 10 µg of MEF1 cell lysate.

Target description

~35 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Affinity

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. technique(s): flow cytometry: suitable, western blot: suitable. NCBI accession no.: NP_598536. UniProt accession no.: Q8BVZ5. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Il33(77125). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.