ANTI-INFLUENZA A VIRUS MATRIX1 CL1G1A12 (C15-1886-988)

General description

Non-structural protein 1 (UniProt: Q4QZ06; also known as NS1) is encoded by the NS1 (also known as NS) gene (Gene ID: 956533) in Influenza A virus. Influenza A virus (IAV) is a highly contagious member of Orthomyxoviridae family. The non-structural protein NS1 is a well conserved protein that is expressed at high levels in infected cells. It is a multifunctional protein associated with various viral functions including mRNA processing, inhibition of cellular apoptosis, limiting interferon production, and inhibition of mRNA splicing. NS1 has two functionally distinct domains: the N-terminal RNA binding domain (RBD) that consists of three -helices; and the C-terminal effector domain that consists of seven -strands and three -helices. The RBD domain binds with several RNA species with low affinity and the effector domain predominantly interacts with host-cell proteins and stabilizes the RBD domain. Clone 2H6 recognizes multiple strains of H5N1 influenza virus. It exhibits high affinity for the RBD of NS1 protein (Kd = 43.5 nM). Threonine 49 (T49) residue in NS1 that forms a direct hydrogen bond with the double stranded RNA is reported to be critical in its interaction with 2H6 antibody. This antibody also reacts with NS1 expressed in virus infected MDCK cells. (Ref.: Barnwal, B., et al. (2015). Antiviral Res. 116; 55-61; Tan, Z., et al. (2010). J. Med. Virol. 82(3); 467-475).

Specificity

Clone 2H6 is a mouse monoclonal antibody that detects non-structural protein 1 (NS1) of the Influenza A virus. It shows reactivity with H1N1, H3N2, and H5N1 strains.

Immunogen

GST-tagged full-length recombinant NS1 protein of Influenza A virus expressed in E.coli.

Application

Quality Control Testing

Evaluated by Western Blotting with recombinant Influenza A virus non-structural (NS1) protein .

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant Influenza A Virus NS1 protein.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Influenza A Virus NS1 protein in Immunofluorescence applications. (Tan, Z., et al. (2010). J Med Virol. 82(3):467-75; Wu, J., et al. (2016). Sci Rep.;6:33382).

Surface plasmon resonance: A representative lot detected Influenza A Virus NS1 protein in Surface plasmon resonance applications. (Barnwal, B., et al. (2015). Antiviral Res. 116:55-61).

Western Blotting Analysis: A representative lot detected Influenza A Virus NS1 protein in Western Blotting applications (Tan, Z., et al. (2010). J Med Virol. 82(3):467-75; Wu, J., et al. (2016). Sci Rep. 6:33382).

Neutralizing: A representative lot of this antibody bound NS1 and reduced influenza A virus replication. (Barnwal, B., et al. (2015). Antiviral Res. 116: 55-61).

ELISA Analysis: A representative lot detected Influenza A Virus NSP1 in ELISA applications (Barnwal, B., et al. (2015). Antiviral Res. 116:55-61; Wu, J., et al. (2016). Sci Rep. 6: 33382).

Inhibition Assay: A representative lot of this antibody inhibited the interaction between NS1 and dsRNA. (Wu, J., et al. (2016). Sci Rep. 6:33382).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Influenza A Virus NSP1, clone 2H6, Cat. No. MABF2218, is a mouse monoclonal antibody that detects NS1 protein and is tested for use in ELISA, Immunofluorescence, Inhibition Assay, Neutralizing, Surface plasmon resonance, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.