Anti-iNOS/NOS II Antibody, NT (C15-1317-524)

General description

Nitric oxide (NO) is an inorganic, gaseous free radical that carries a variety of messages between cells. Vasorelaxation, neurotransmission and cytotoxicity can all be potentiated through cellular response to NO. NO production is mediated by members of the nitric oxide synthase (NOS) family. NOS catalyzes the oxidization of L-arginine to produce L-citrulline and NO. Two constitutive isoforms, brain or neuronal NOS (b or nNOS, type I) & endothelial cell NOS (eNOS, type III), and one inducible isoform (iNOS, type II), have been cloned. Cytokines such as interferon-gamma (IFN), tumor necrosis factor (TNF), interleukin-1 and -2, and lipopolysaccarides (LPS) cause an increase in iNOS mRNA, protein, and activity levels. Protein kinase C-stimulating agents exhibit the same effect on iNOS activity. Human iNOS is regulated by calcium/calmodulin (in contrast with mouse NOS2).

Specificity

Other homologies: Rat (85% sequence homology).

This antibody recognizes iNOS/NOS II at the N-terminus.

Immunogen

Epitope: N-terminus

GST-tagged recombinant protein corresponding to the N-terminus of mouse iNOS/NOS II.

Application

Detect iNOS/NOS II using this Anti-iNOS/NOS II Antibody, NT validated for use in WB, IP, IH(P).

Immunohistochemistry Analysis: 1:50-200 dilution from a representative lot detected iNOS/NOS II in malignant human lung tissues.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated iNOS/NOSII from IFNgamma/LPS treated RAW264.7 cell lysate.

Research Category
Neuroscience

Research Sub Category
Oxidative Stress

Quality

Evaluated by Western Blot in IFNgamma/LPS untreated and treated RAW264.7 cell lysates.

Western Blot Analysis: 0.5 µg/mL of this antibody detected iNOS/NOS II on 10 µg of IFNgamma/LPS untreated and treated RAW264.7 cell lysates.

Target description

~125 kDa observed

Linkage

Replaces: 06-573

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
IFNgamma/LPS untreated and treated RAW264.7 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human. packaging: antibody small pack of 25 . μ. g. technique(s): immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_035057. UniProt accession no.: P29477. shipped in: ambient. storage temp.: 2-8°C. target post-translational modification: unmodified. Gene Information: mouse ... Nos2(18126).