Specificity
Reacts with the I domain (Val151 - Ala364) of human alpha1 integrin (Fabbri, 1996). Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi, 1991). Does not recognize recombinant I domain of rat integrin alpha1.
Application
Detect Integrin α1 using this Anti-Integrin α1 Antibody, clone FB12, azide free validated for use in ELISA, FC, IH, IP, FUNC & WB.
Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation. Immunoblotting: 2 μg/mL
Immunoprecipitation: 1 μg/mL
FACS analysis: 2 μg/mL
ELISA: 0.5 μg/mL
Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri, 1996): 1-5 μg/mL
Optimal working dilutions must be determined by end user.
Target description
130 kDa
Physical form
Format: Purified
Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl.
Analysis Note
Control
Microvascular endothelium
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
- UPC:
- 51203401
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- MAB1973Z
- Product Size:
- 100/µG
