Specificity
Human alpha2 integrin subunit. Specificity determined by immunoprecipitation from a detergent extract of 35S methionine labeled endothelial cells. The epitope recognized is on the cytoplasmic domain of the protein and is thus intracellular. Shows no cross-reactivity to alpha1, alpha3, alpha4, alpha5, alpha6, or alphaV subunits.
Immunogen
Synthetic peptide derived from the COOH terminal sequence (cytoplasmic domain) of the human alpha2 integrin subunit {KYEKMTKNPDEODETTELSS}
Application
Anti-Integrin α2 Antibody detects level of Integrin α2 & has been published & validated for use in ELISA, IF, IH, IP, RIA & WB.
Immunofluorescence: 1:500 (suggested for use on acetone-fixed tissue only)
ELISA/RIA: 1:1000
Western blot: 1:1000 The antibody has been used to report alpha2 expression in HUVECs during tubule-like structure formation in collagen (Deroanne, 1996). Recognizes ~160kDa alpha 2 subunit
Immunoprecipitation: 5 μL precipitates alpha2/beta1 from 5 x 106 cells.
Immunohistochemistry: 1:500
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
Target description
160kDa
Physical form
Neat rabbit antiSerum. Liquid with 0.05% sodium azide
Unpurified
Storage and Stability
Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Skin (basement membrane), High grade ovarian tumors
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: chicken, rat, hamster, mouse, human. manufacturer/tradename: Chemicon®. . technique(s): ELISA: suitable, immunofluorescence: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, radioimmunoassay: suitable, western blot: suitable. NCBI accession no.: NM_002203.3. UniProt accession no.: P17301. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... ITGA2(3673). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.- UPC:
- 51343805
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- AB1936
