Anti-Integrin beta-3 Antibody (C15-1315-997)

General description

Integrin beta-3 is a member of the Integrin receptor family. The integrin family has been shown to be instrumental in cell adhesion to extracellular matrix proteins and can be found in certain cell to cell adhesions. Integrins are also important in transmembrane connections and intracellular signaling. Integrins are key to understanding many human diseases as they are commonly the receptors. Integrins have been linked to many different parts of cellular operations including; development, immunity, leukocyte traffic, homeostasis and even cancer. Once integrin beta-3 is activated, it binds fibrinogen which causes platelet to platelet interaction effectively cutting off a ruptured endothelial surface.

Specificity

Other homologies: Mouse (93% sequence homology.)

This antibody recognizes the extracellular domain of integrin beta-3.

Immunogen

Epitope: Extracellular Domain

KLH-conjugated linear peptide corresponding to human integrin beta-3 at the extracellular domain.

Application

Anti-Integrin beta-3 Antibody is an antibody against Integrin beta-3 for use in WB, IP, IH(P).

Immunohistochemistry Analysis: A 1:500 dilution from a previous lot detected intergin beta-3 in skin membrane tissue.

Immunoprecipitaiton Analysis: 10 µg from a previous lot immunoprecipitated integrin beta-3 from U251 RIPA lysate.

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Quality

Evaluated by Western Blot in U251 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected integrin beta-3 in 10 µg of U251 cell lysate.

Target description

~85 kDa observed.
Uncharacterized bands may be observed in some lysates.

Linkage

Replaces: 04-1060

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
U251 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, pig. species reactivity (predicted by homology): rat (based on 100% sequence homology), canine (based on 100% sequence homology), porcine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology). technique(s): immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_000203. UniProt accession no.: P05106. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... ITGB3(3690). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.