Anti-Interferon-beta Antibody (C15-1315-711)

General description

Interferon beta is a globular protein consisting of 5 alpha helices with a predicted molecular weight of 20 kDa. Due to glycosylation, however, the observed molecular weight is closer to 25 kDa by SDS-PAGE.

Specificity

Recognizes Human interferon beta.

Immunogen

Recombinant human interferon beta expressed in E. coli

Application

Interferon neutralization: 2 x 10e5 neutralization units/mL where one neutralization unit is the amount of antiserum required to neutralize one unit of human interferon beta (Hu-IFN-b) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," in J. Virol., Vol. 37, 755-758]; [Familletti, P.C., Rubinstein, S., and Pestka, S. (1981) "A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon," in Methods of Enzymology, Vol. 78 (S. Pestka, ed.), Academic Press, New York, 387-394]. In this antiviral assay for interferon about 1 unit/mL of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for human interferon beta provided by the National Institutes of Health [see Pestka, S. (1986) "Interferon Standards and General Abbreviations," in Methods in Enzymology (S. Pestka, ed.), Academic Press, New York 119, 14-23]. This material is prepared specifically for the effective neutralization of human interferon beta.

Immunohistochemistry

Western blot: 1:5000

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

This Anti-Interferon-β Antibody is validated for use in ELISA, IH, IP, NEUT, WB for the detection of Interferon-β.

Target description

20 kDa

Linkage

Replaces: 04-1095

Physical form

Rabbit Serum diluited with PBS. Liquid containing no preservatives.

Unpurified

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
IFN expressing cell lines

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human. manufacturer/tradename: Chemicon^®. . technique(s): ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, neutralization: suitable, western blot: suitable. NCBI accession no.: NM_000600.1. UniProt accession no.: P05231. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... IFNB1(3456). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.