Anti-Interleukin-1beta antibody produced in goat

General description

Interleukins are proinflammatory cytokines produced on cell injury or trauma. There are two closely related types of interleukins produced by the cell, IL-1α and IL-1β that are produced by the activation of NF-κ B transcription factor in response to bacterial infection or LPS. Both IL-1α and IL-1β exert their cellular effects by signalling through IL-1 receptor type 1. IL-1β has pleiotrophic activities and is protective against infections by recruitment of neutrophils to the infection site, secretion of cytokines and chemokines and activation of adhesion molecules. IL-1β overproduction has been implicated in many auto-immune and allergic disorders such as Cryopirin-associated periodic syndromes, Muckle-Wells syndrome and skin lesions
Anti-Interleukin-1β antibody specifically reacts with mouse IL-1β. The antibody shows 25% cross reactivity with human recombinant IL-1β.

Specificity

The antibody will neutralize the biological activity of recombinant mouse IL-1β, but not recombinant human IL-1β. The antibody shows ~25% cross-reactivity with recombinant human IL-1β.

Immunogen

recombinant mouse IL-1β.

Application

Anti-interleukin-1β may be used to neutralize the biological activity of recombinant mouse IL-1β on mouse T-helper D10.G4.1 cell line at a concentration of 50 picog/ml. No neutralization of the biological activity of recombinant human IL-1β is observed. The antibody may be used for immunoblotting at a working concentration 1-2 μg/ml or a dilution of 1:1000. In immunoblotting applications the antibody shows 25% cross-reactivity with human recombinant IL-1β.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Preparation Note

Purified using protein G.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.