General description
Inositol-requiring protein 1 (IRE1p), also known as Ire1-alpha (IRE1a), Endoplasmic reticulum-to-nucleus signaling 1 (ERN1), and Serine/threonine-protein kinase/endoribonuclease IRE1, contains 1 KEN domain and 1 protein kinase domain.
IRE1p has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1p is ubiquitously expressed with high levels observed in pancreatic tissue.
Specificity
This antibody recognizes the C-terminus of IRE1p.
Immunogen
Epitope: C-terminus
KLH-conjugated linear peptide corresponding to the C-terminus of human IRE1p.
Application
Detect Inositol-requiring protein 1 using this rabbit polyclonal antibody, Anti-IRE1p Antibody validated for use in western blotting, ICC & IHC.
Immunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected IRE1p in human pancreas and kidney tissues.
Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected IRE1p in A20 cells.
Immunofluorescence Analysis: 2 µg/mL from a representative lot detected IRE1p in A20 cells.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Quality
Evaluated by Western Blotting in A20 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected IRE1p in A20 cell lysate.
Target description
~114 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
A20 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human. technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: O75460. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... ERN1(2081). Storage Class Code: 10 - Combustible liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 51313901
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABN464