Anti-IRX-3 (C15-1317-689)

General description

Iroquois-class homeodomain protein IRX-3 (UniProt P81067; also known as Homeodomain protein IRXB1, Iroquois homeobox protein 3) is encoded by the Irx3 (also known as Irxb1) gene (Gene ID 16373) in murine species. Iroquois homeobox (Irx) genes encode a family of homeodomain transcription factors that are conserved from worms to vertebrates. Six IRX genes are located in two clusters in human (IRXA and IRXB) and mouse (IrxA and IrxB) genomes. Irx3 and Irx5 of the IrxB cluster are expressed in the developing mouse limb bud and are essential for proximal and anterior skeletal formation. Irx3 and Irx5 are required to establish early anteroposterior (AP) polarity and specify the anterior progenitor population. Individual Irx genes are also important for certain postnatal cardiac functions in the developing mouse heart, including cardiac repolarization (Irx5) and rapid ventricular conduction (Irx3). Postnatal deletion of Irx3 and Irx5 in the myocardium leads to prolongation of atrioventricular conduction, due in part to activation of expression of the Na(+) channel protein Nav1.5. However, combined postnatal loss of Irx3 and Irx5 results in a restoration of the repolarization gradient that is altered in Irx5 mutant hearts, suggesting that postnatal Irx3 activity can be repressed by Irx5 in mice.

Specificity

Expected to react with both spliced isoforms of murine IRX-3/IRXB1 reported by UniProt (P81067).

Immunogen

Recombinant murine IRX-3/IRXB1 C-terminal fragment.

Application

This rabbit polyclonal Anti-IRX-3 Antibody, Cat. No. ABS1050 detects levels of IRX-3, and has been published and validated for use in Western Blotting.

Western Blotting Analysis: A 1:5,000 dilution from a representative lot detected IRX-3/IRXB1 in 10 µg of embryonic E16 and postnatal (P1 and P7) mouse brain tissue lysates.

Western Blotting Analysis: A representative lot was affinity purified and detected a complete elimination of IRX-3/IRXB1 expression in hindlimb bud lysates of embryos from pregnant Cre-ER(TM);Irx3/5-CKO mice 48 hours after Cre induction by tamoxifen treatment (Li, D., et al. (2014). Dev. Cell. 29(2):233-240).

Western Blotting Analysis: A representative lot was affinity purified and detected both endogenous IRX-3/IRXB1 and tissue-specific expression of a dominant negative EnR-Irx3 transgene in the hypothalamus tissue lysate from EnR-Irx3;Ins2-Cre mice (Smemo, S., et al. (2014). Nature. 507(7492):371-375).

Quality

Evaluated by Western Blotting in embryonic mouse brain tissue lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antiserum detected IRX-3/IRXB1 in 10 µg of E13-14 embryonic mouse brain tissue lysate.

Target description

~58 kDa observed. 52.69 kDa (isoform 1) and 54.89 kDa (isoform 2) calculated. Uncharacterized bands may be observed in some lysate(s).

Other Notes

Concentration: Please refer to lot specific datasheet.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse. technique(s): western blot: suitable. NCBI accession no.: NP_001240751. UniProt accession no.: P81067. shipped in: ambient. target post-translational modification: unmodified. Gene Information: mouse ... Irx3(16373). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.