Anti-ISL-1 Antibody, clone 16E8.1

General description

Islet-1 (ISL-1) is a transcription factor that plays an important role in the embryogenesis of pancreatic islets of Langerhans. It is expressed in subsets of neurons of the adrenal medulla and dorsal root ganglion, inner nuclear and ganglion cell layers in the retina, the pineal and some regions of the brain. In mouse embryos, a deficiency of this gene fail to undergo neural tube motor neuron differentiation. More recently it has been identified as a marker for a cardiac progenitor cell lineage that is capable of differentiating into all 3 major heart cell types: cardiomyocytes, smooth muscle and endothelial cell lineages.

Immunogen

GST-tagged linear peptide corresponding to Human ISL-1.

Application

Evaluated by Immunohistochemistry in both human cerebellum and rat hippocampal tissue. Immunohistochemsitry Analysis: A 1:1000 dilution of this antibody detected ISL-1 in both human cerebellum and rat hippocampal tissue.

Research Category
Stem Cell Research

Research Sub Category
Pluripotent & Early Differentiation

This Anti-ISL-1 Antibody, clone 16E8.1 is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of ISL-1.

Quality

Evaluated by Western Blot in a K562 cell lysate. Western Blot Analysis: A 1μg/ml (1:1000) dilution of this antibody detected ISL-1 in a K562 cell lysate.

Target description

~ 40 kDa observed

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.