ANTI-JAM-A ANTIBODY CLONE BV11

General description

Junctional adhesion molecule A (JAM-A) is found in endothelial and epithelial tight junctions and is involved in the inflammatory enlistment of mononuclear cells containing LFA-1. JAM-A is linked to atherogenesis, platelet activation, transmigration of leukocytes and angiogenesis. JAM-A is also found to be important in the development of many different organ systems, and is mostly expressed in the epithelial parts and the embryonic vasculature. JAM-A is indicated to play a role in epithelial permeability, inflammation and proliferation in the intestine, aiding in homeostasis.

Immunogen

H5V murine heart capillary endothelial cells.

Application

Detect JAM-A using this Anti-JAM-A Antibody, clone BV11 validated for use in FC, IC & IP.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in cell extracts from Mouse H5V and PDV cells, and in COS cultures transfected with full length cDNA of mouse JAM-A (Martin-Padura, I., et al. (1998). J Cell Biol. 142(1):117-127).

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in cultured epithelial cells, cremaster muscles, and Schwann Cells (Martin-Padura, I., et al. (1998). J Cell Biol. 142(1):117-127).

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

Quality

Evaluated by Flow Cytometry in bEnd.3 cells.

Flow Cytometry Analysis: A 1:1,000 dilution of this antibody detected JAM-A in bEnd.3 cells.

Target description

32 kDa calculated

Physical form

Format: Purified

Protein G Purified

Purified rat monocolonal IgG2bκ in buffer containing PBS and no preservatives.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
bEnd.3 cells

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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