Anti-KDEL Mouse mAb (10C3) (C15-1303-444)

General description

Protein G purified mouse monoclonal antibody. Recognizes the KDEL Grp78 (BiP) and Grp94 proteins.

Recognizes the Grp78 (BiP) and Grp94 proteins.

This Anti-KDEL Mouse mAb (10C3) is validated for use in Immunoblotting, Immunocytochemistry, Immunoprecipitation for the detection of KDEL.

Immunogen

Rat

a synthetic peptide (SEKDEL) corresponding to amino acids 649-654 of rat Grp78, conjugated to KLH

Application

Immunoblotting (4 µg/ml, colorimetric)

Immunocytochemistry (5 µg/ml)

Immunoprecipitation (5 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Highly Toxic (H)

Physical form

In PBS, 50% glycerol.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
RK-13 cells, Mouse liver microsomes, Rat liver microsomes, Heat shocked HeLa cells

Other Notes

Also recognizes unidentified proteins at ~55 kDa and ~43 kDa by immunoblotting. Does not cross-react with Hsp70. Variables associated with assay conditions will dictate the proper working dilution.

Kuznetsov, G., et al. 1994. J. Biol. Chem.269, 22990.
Huovila, A.-P., et al. 1992. J. Cell Biol. 118, 1305.
Munro, S., and Pelham, H.R.B. 1987. Cell48, 899.
Munro, S., and Pelham, H.R.B. 1986. Cell46, 291.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: mouse. Quality Level: 100. antibody form: purified antibody. antibody product type: primary antibodies. clone: 10C3, monoclonal. form: liquid. contains: ≤. 0.1% sodium azide as preservative. species reactivity: mouse, sheep, canine, hamster, Drosophila, rat, guinea pig, human, monkey, yeast, rabbit, porcine. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG2a. shipped in: wet ice. storage temp.: −. 20°C. target post-translational modification: unmodified. Gene Information: mouse ... Kdelr1(68137). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.