ANTI-KEAP1 ANTIBODY (C005B-241749)

General description

Anti-oxidant defense is known to be regulated by various mechanisms including the usage of nuclear factor erythroid 2-related factor 2 (Nrf2). Under normal physiological conditions, Nrf2 is bound to Kelch-like ECH-associated protein 1 (Keap1), a cytoplasmic repressor. Keap1 also acts as a substrate adaptor for Cullin3-dependent ubiquitin ligase and also targets Nrf2 for degradation by proteosomes. The substrate adaptor function of Keap1 is inactivated during physiological stress. In this respective condition, a range of oxidative and electrophilic stimuli such as ROS, diethyl malonate, and certain disease processes, resulting from Nrf2 accumulation, enters the nucleus and thereby activates the expression of select anti-oxidant genes. Such genes promote the detoxification of ROS and other harmful molecules, which contribute to the carcinogenesis.

Specificity

This antibody recognizes the Kelch 3 region of KEAP1.

Immunogen

Epitope: Kelch 3 Region

KLH-conjugated linear peptide corresponding to the Kelch 3 region of human KEAP1.

Application

Anti-KEAP1 Antibody detects level of KEAP1 & has been published & validated for use in WB & IC.

Immunocytochemistry Analysis: 2 µg/mL from a representative lot detected KEAP1 in A431 and HeLa cells.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
General Post-translation Modification

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected KEAP1 in 10 µg of HeLa cell lysate.

Target description

~60-70 kDa observed.
A doublet may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HeLa cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, human, rat. technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_036421. UniProt accession no.: Q14145. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... KEAP1(9817). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.

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