Anti-Kinectin Antibody (C15-1318-085)

General description

Organelle transport is facilitated by molecular motors, such as kinesin and dynein, which interact with microtubules to transport cargo in an ATP-dependent manner. The shuttling of organelles is also dependent on kinectin, a transmembrane ER protein that associates with kinesin and stimulates its ATPase activity. Kinesin-kinectin dynamics have also been linked to focal adhesion growth. In addition, kinectin has been shown to activate the RhoG GTPase pathway which involves Rac1 and Cdc42 signaling. Kinectin is expressed in testis, ovary, spleen, thymus, prostate, colon, and small intestine.

Specificity

Other homologies: Mouse (82% Mouse sequence homology) and Rat (79% sequence homology).

This antibody recognizes the lumenal region of Kinectin.

Immunogen

Epitope: Lumenal region

KLH-conjugated linear peptide corresponding to the lumenal region of human Kinectin.

Application

Detect Kinectin using this Anti-Kinectin Antibody validated for use in Western Blotting, ICC.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Kinectin in HeLa and A431 cells.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Kinectin in 10 µg of human testis tissue lysate.

Target description

~158 kDa observed. Uniprot describes 3 isoforms at ~149, ~150, and ~156 kDa

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human testis tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. species reactivity (predicted by homology): rhesus macaque (based on 100% sequence homology). technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_001072989. UniProt accession no.: Q86UP2. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... KTN1(3895). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.