ANTI-KININOGEN-1 CLONE 2B7 (C005B-449472)

General description

Ankyrin repeat and sterile alpha motif domain-containing protein 1B (UniProt: P0C6S7; also known as Amyloid-beta protein intracellular domain-associated protein 1, AIDA-1, E2A-PBX1-associated protein, EB-1) is encoded by the Anks1b gene (Gene ID: 314721) in rat. AIDA-1 is an adaptor protein that has been implicated in Cajal body and nucleolar function. It is expressed throughout the brain and is highly expressed in the hippocampus. It is one of the most abundant proteins at postsynaptic densities where it is reported to bind to PSD95 and associates with NMDA receptors (NMDARs). In the brain, it interacts with amyloid b precursor protein and plays a role in brain development. AIDA-1 preferentially associates with GluN2B-containing NMDARs and plays a role in their transport from the endoplasmic reticulum (ER). Loss of AIDA-1 results in the accumulation of GluN2B in the ER and decreases GluN2B and increases GluN2A distribution at the synapse. However, loss of AIDA-1 does not affect AMPAR-mediated synaptic transmission or the probability of glutamate release. AIDA-1 has seven Ankyrin repeats in its N-terminal half and has two closely juxtaposed sterile alpha motif (SAM) domains (aa 810-876 and 884-949). Within the second SAM domain resides the nuclear localization signal (aa 935-938). Mutations in Anks1b gene are known to affect GluN2B function and are linked to the pathogenesis of neuropsychiatric disorders. (Ref.: Carbonell, AU., et al., (2019). Nat. Commun. 10(1); 3529; Tindi, JO., et al. (2015). J. Neurosci. 35(24); 8986- 8996).

Specificity

Clone 2K9 is a mouse monoclonal antibody that detects AIDA1/ANKS1B. It targets an epitope within 10 amino acids from the C-terminal half.

Immunogen

A linear peptide corresponding to 10 amino acids from the SAM1/2 domain in the C-terminal half of rat AIDA1/ANKS1B.

Application

Quality Control Testing

Evaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected AIDA1 in Rat brain tissue lysate (non-reduced).

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected AIDA1 in rat testis tissue lysates (non-reduced).

Immunocytochemistry Analysis: A representative lot detected AIDA1 in Immunocytochemistry applications (Tindi, J.O., et al. (2015). J Neurosci. 35(24):8986-96).

Western Blotting Analysis: A representative lot detected AIDA1 in Western Blotting applications (Tindi, J.O., et al. (2015). J Neurosci. 35(24):8986-96).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-AIDA1, clone 2K9, Cat. No. MABN2638, is a mouse monoclonal antibody that detects AIDA1 and is tested for use in Immunocytochemistry and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.