ANTI-KININOGEN-1 CLONE 3E8 (C005B-449494)

General description

cGMP-gated cation channel alpha-1 (UniProt: Q00194; also known as Cyclic nucleotide-gated cation channel 1, Cyclic nucleotide-gated channel alpha-1, CNG channel alpha-1, CNG-1, CNG1, Cyclic nucleotide-gated channel, photoreceptor, Rod photoreceptor cGMP-gated channel subunit alpha) is encoded by the CNGA1 (also known as CNCG, CNCG1) gene (Gene ID: 281700) in bovine species. CNGA1 is a multi-pass membrane protein with four cytoplasmic domains, six transmembrane domains, and three extracellular domains. It is a subunit of the rod cyclic GMP-gated cation channel that is localized to the rod outer segment and is involved in the final stage of the phototransduction pathway. It forms a heterotetramer with CNGB1 (in a 3;1 ratio) but may also form homotetrameric channels that are strongly activated by saturating cGMP. When light hits rod photoreceptors, cGMP levels decrease that results in rapid closure of CNGA1/CNGB1 channels and hyperpolarization of the membrane potential. In this channel, CNGA1 forms a central channel gate in the middle of the membrane and occludes the central cavity. CNGA1 has two structural components: the membrane-spanning S1-S6 region and the cytosolic ligand gating apparatus. The transmembrane S1-S6 region of each subunit is separated into an S1-S4 voltage-sensor domain and an S5-S6 pore domain. The cytosolic soluble fragment of CNGA1 forms the gating apparatus in a channel tetramer that is responsible for cGMP activation of the channel. (Ref.: Xue, J., et al. (2021). Neuron. 109(8); 1302-1313; Cook, NJ., et al. (1989). J. Biol. Chem. 264(12); 6996-6999).

Specificity

Anti-CNGA1/CNG1, clone PMc 1D1, Cat. No. MABN2617, is a mouse monoclonal antibody that detects cGMP-gated cation channel alpha-1 (CNGA1) and is tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Immunogen

Plasma membrane preparation purified from bovine rod outer segments.

Application

Quality Control Testing

Evaluated by Immunohistochemistry (Paraffin) in mouse retina tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected CNGA1/CNG1 in mouse retina tissue sections.

Tested Applications

Immunohistochemistry Applications: A representative lot detected CNGA1/CNG1 in Immunohistochemistry applications (Sharif, A.S., et al. (2018). J Neurosci. 38(13):3160-3176).

Immunoprecipitation Analysis: A representative lot detected CNGA1/CNG1 in Immunoprecipitation applications (Cheng, C.L., et al. (2013). J Cell Sci. 126(Pt24):5725-34).

Western Blotting Analysis: A representative lot detected CNGA1/CNG1 in Western Blotting applications (Cook, N.J., et al. (1989). J Biol Chem. 264(1):6996-9; Cheng, C.L., et al. (2013). J Cell Sci. 126(Pt24):5725-34).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

This mouse monoclonal Anti-CNGA1/CNG1, clone PMc 1D1, Cat. No. MABN2617 is tested for use in Immunohistochemistry (Paraffin), Western Blotting, Immunoprecipitation, for the detection of CNGA1.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.