Anti-KLF4 Antibody (C15-1316-148)

Specificity

Recognizes KLF4. The calculated molecular weight is ~50.1 kDa.

Immunogen

Synthetic peptide corresponding to amino acids 28-38 of human and mouse KLF4 (AGAPNNRWREE).

Application

Anti-KLF4 Antibody is a Rabbit Polyclonal Antibody for detection of KLF4 also known as Kruppel-like Factor 4 & has been validated in ICC.

Immunocytochemistry: 1/200 to 1/1000.

Optimal dilutions must be determined by the user.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Physical form

Affinity purified immunoglobulin precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.

Storage and Stability

Maintain unopened vial at -20°C for up to 6 months. Avoid repeated freeze/thaw cycles.

The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C.

For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality.

For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles.

PREPARATION AND USE:

To reconstitute the antibody, centrifuge the antibody vial at moderate speed (5,000 rpm) for 5 minutes to pellet the precipitated antibody product. Carefully remove the ammonium sulfate/PBS buffer solution and discard. It is not necessary to remove all of the ammonium sulfate/PBS solution: 10 μL of residual ammonium sulfate solution will not effect the resuspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur.

Resuspend the antibody pellet in any suitable biological buffer, standard PBS or TBS (pH 7.3-7.5) are typical. Volumes required are not critical but it is suggested that the final antibody concentration be between 0.1 mg/mL and 1.0 mg/mL. For example, to achieve a1 mg/mL concentration with 50 μg of precipitated antibody, the amount of buffer needed would be 50 μL.

Carefully add the liquid buffer to the pellet. DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to resuspend are normal. Vials are overfilled to compensate for any losses.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100, 300. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse, rat, human. manufacturer/tradename: Chemicon^®. . technique(s): immunocytochemistry: suitable. NCBI accession no.: NM_004235.3. UniProt accession no.: O43474. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... KLF4(9314).