ANTI-KV12.1 ANTIBODY (C15-1316-579)

Specificity

Recognizes Kv12.1 (Elk1, KCNH8, ether-a-go-go like K⁺ channel 3, Voltage gated K⁺ channel subfamily H member 8).

The immunogen sequence is 14 of 15 amino acids identical in mouse.

Immunogen

Purified peptide from amino acids 143-157 of rat Kv12.1 .

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-Kv12.1 Antibody is validated for use in WB for the detection of Kv12.1.

Western blot: 1:200 using ECL on rat cortex lysate.
Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Physical form

Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, and 0.025% sodium azide as a preservative. Reconstitute with 200 µL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).

ImmunoAffinity Purified

Storage and Stability

Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Included free of charge with the antibody is 40 µg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 µL of sterile deionized water. For negative control, preincubate 1 µg of peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity purified immunoglobulin. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human, rat. manufacturer/tradename: Chemicon^®. . technique(s): western blot: suitable. NCBI accession no.: NM_145095. UniProt accession no.: Q9QWS8. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... KCNH8(131096). Hazard Statements: H412. Precautionary Statements: P273 - P501. Hazard Classifications: Aquatic Chronic 3. Storage Class Code: 11 - Combustible Solids. WGK: WGK 3.

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