ANTI-LAMC2. CLONE P2H 100 Y56

General description

Laminin subunit g2 (UniProt: Q13753; also known as Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as LAMB2T, LAMNB2) gene (Gene ID: 3918) in human. Laminin is a major basement membrane glycoprotein consisting of α, β, γ chains that are bound to each other by disulfide bonds. Over 15 different isoforms have been reported in the laminin family that are expressed in a tissue-specific manner and play different role in each tissue. γ², a subunit of Laminin-5 is reported to be frequently over-expressed as a monomer or as the β²−γ³ heterodimer in invasive cancers and is regarded as an invasion marker. It has 8 EGF-like domains. The cell adhesion and motility activities of Laminin-5 are reported to be regulated by the proteolytic cleavage of the short arm of Laminin γ² chain by BMP-1 or MT1-MMP. This cleavage releases a 45 kDa N-terminal proteolytic fragment (γ²pf). The short arm and γ²pf can be further cleaved by serine proteases and by matrix metalloproteinases resulting in the release of small N-terminal fragments. γ²pf-like fragments have been reported to be present in conditioned media of cultured cancer cells and in sera from human cancer subjects. Clone P2H targets the second N-terminal EGF-like repeat (NE2) of domain V of laminin 2. (Ref.: Sato, H., et al. (2014). Cancer Sci. 105(2); 168-175; Miyazaki, K., et al. (2016). Cancer Sci. 107(12); 1909-1918).

Specificity

Clone P2H is a mouse monoclonal antibody that detects Laminin-5, g2 chain. It targets an epitope within the N-terminal, NE2 in domain V.

Immunogen

His-tagged 45-kDa N-terminal fragment consisting of g2pf, domain V.

Application

Quality Control Testing

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Laminin-5 subunit g2 (LAMC2) in A431 cells.

Tested Applications

Western Blotting Analysis: 1 μg/mL from a representative lot detected LAMC2 in lung squamous cell carcinoma cell lysates (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Western Blotting Analysis: A representative lot detected LAMC2 in Western Blotting applications (Miyazaki, K., et. al. (2016). Cancer Sci. 107(12):1909-1918).

Immunohistochemistry (Paraffin) Analysis: 10 μg/mL from a representative lot detected LAMC2 in normal human skin, skin cancer, normal breast, and breast adenocarcinoma tissues (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

ELISA Analysis: 5 μg/mL from a representative lot detected LAMC2 in Lm-gamma2pf and Lm-gamma2 in Lm332 condition medium from Lm332-expressing HEK293 cells (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Immunocytochemistry Analysis: A representative lot detected LAMC2 in Immunocytochemistry applications (Miyazaki, K., et. al. (2016). Cancer Sci. 107(12):1909-1918).

Immunohistochemistry Applications: A representative lot detected LAMC2 in Immunohistochemistry applications of lung squamous cell carcinomas and lung adenocarcinomas (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-LAMC2, clone P2H, Cat. No. MABT1460, is a mouse monoclonal antibody that detects Laminin subunit g2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.