ANTI-LAMIN B RECEPTOR (LBR) CLONE BB2SS3F3 (C15-1884-856)

General description

Lamin-B receptor (UniProt: Q14739; also known as Integral nuclear envelope inner membrane protein, LMN2R) is encoded by the LBR gene (Gene ID: 3930) in human. Lamin-B receptor is a multi-pass membrane protein that anchors the lamina and the heterochromatin to the inner nuclear membrane. It is an integral membrane protein of the interphase nuclear envelope (NE). It contains a Tudor damain that lies within the first 62 amino acids. Its N-terminal end resides in the nucleoplasm, binding to lamin B and heterochromatin and the C-terminal end resides within the inner nuclear membrane, retreating with the endoplasmic reticulum (ER) away from condensing chromosomes during mitotic NE breakdown. During mitosis, Lamin-B receptor is retained within the retreating ER and kept out of the way of the mitotic apparatus. Lamin- B receptor is suggested to perform three vital functions in the cell: (a) contributes to post-mitotic nuclear reformation; (b) contributes to interphase NE growth; (c) contributes to compartmentalization of heterochromatin within the interphase nucleus. Lamin-B receptor is phosphorylated by Cdk1 in mitosis when the inner nuclear membrane breaks down into vesicles that dissociate from the lamina and the chromatin. It is phosphorylated by different protein kinases in interphase when the membrane is associated with these structures. Mutations in LBR gene are known to cause Pelger-Huet anomaly that is characterized by skeletal anomalies, developmental delay, and seizures. Some mutations cause Greenberg dysplasia that can lead to in utero lethality. (Ref.: Olins, AL., et al. (2010). Nucleus 1(1); 53-70).

Specificity

Clone BB2SS3F3 detects Lamin B receptor in human cells. It targets an epitope within the first 211 amino acids.

Immunogen

GST-tagged recombinant fragment corresponding to the first 211 amino acids from human Lamin B receptor.

Application

Anti-Lamin B Receptor (LBR), clone BB2SS3F3, Cat. No. MABT831, is a mouse monoclonal antibody that detects Lamin B receptor and has been tested for use in Immunocytochemistry, Immunofluorescence, and Western Blotting.

Immunofluorescence Analysis: A representative lot detected Lamin B Receptor (LBR) in C57BL/6 mouse small intestine (Courtesy of Dr. Brian Burke, Institute of Medical Biology, A*STAR, Singapore).

Western Blotting Analysis: A representative lot detected Lamin B Receptor (LBR) in HeLa cell lysate, mouse C2C12 cell lysate, and in adult mouse fibroblasts (MAFs) either LBR+/+ or LBR -/- (Courtesy of Dr. Brian Burke, Institute of Medical Biology, A*STAR, Singapore).

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected Lamin B Receptor (LBR) in HeLa cells.

Immunocytochemistry Analysis: A representative lot detected Lamin B Receptor (LBR) in HeLa and NIE-115 cells, as well as in mouse adult fibroblasts (MAF LBR+/+ vs MAF LBR-/-) (Courtesy of Dr. Brian Burke, Institute of Medical Biology, A*STAR, Singapore).

Research Category
Cell Structure

Quality

Evaluated by Western Blotting in mouse spleen tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Lamin B Receptor (LBR) in 10 µg of mouse spleen tissue lysate.

Target description

~55 kDa observed; 70.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.