ANTI-LAMP-2 ANTIBODY CLONE ABL-93

General description

Lysosome-associated membrane protein 2 (LAMP-2) is a highly glycosolated protein belonging to the lysosome-associated membrane protein family. This group pf proteins composes a significant portion of the lysosomal membrane and are considered crucial mediators for cellular adhesion by interacting with carbohydrate receptors. LAMP-2 is a profuse and critical component of the lysosomal membrane as it has been implicated in playing a role in lysosomal biogenesis. It also appears to be involved in later stages of phagocytosis and autophagy, where it may act to protect the lysosomal membrane from being digested, assist in signal transduction, moderate levels of acidity within the lysosomal environment, and participate in adhesion. Mutations in LAMP-2 expression have been associated with lysosomal storage diseases or LSDs, along with Danon disease, an X-linked disorder characterized by skeletal myopathy, mental retardation, and hypertrophic cardiomyopathy.

Specificity

This antibody recognizes LAMP-2.

Immunogen

Purified glycoproteins from NIH/3T3 cells, corresponding to mouse LAMP-2.

Application

Anti-LAMP-2 Antibody, clone ABL-93 is an antibody against LAMP-2 for use in WB, IP & IF.

Immunoprecipitation Analysis: This antibody has been shown to immunoprecipitate LAMP-2 in HaNIH cells, (Chen, 1985).

Immunofluorescence Analysis: This antibody has been shown to detect LAMP-2 in mouse brain, (Ferguson, 2009).

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected LAMP-2 in 10 µg of NIH/3T3 cell lysate.

Target description

~ 80 kDa observed MW. The calculated MW is 45 kDa LAMP-2 is heavily glycosoated and has been routinely observed at MW′s of 80-120 kDa based on publication data, (Chen, 1985; Ferguson, 2009).

Physical form

Format: Purified

Protein G

Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
NIH/3T3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.