ANTI-LCN13 ANTIBODY (C005B-241667)

General description

Epididymal-specific lipocalin-13 (LCN13) is also called Odorant-binding protein 2a (Obp2a). LCN13 is a secreted protein that is thought to act as a retinoid carrier protein within the epididymis, and have a role in male fertility. LCN13 is expressed in the epididymis.

Specificity

This antibody detects two observed isoforms of LCN13.

Immunogen

Recombinant protein corresponding to mouse LCN13.

Application

Detect Lipocalin using this rabbit polyclonal antibody, Anti-LCN13 Antibody validated for use in western blotting.

Research Category
Metabolism

Research Sub Category
Lipid Metabolism & Weight Regulation

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LCN13 in mouse lung tissue lysate.

Western Blotting Analysis: A representative lot from an independent laboratory detected LCN13 in liver, muscle, and pancreas tissues, but not in white adipose tissue, from LCN13 transgenic male mice (Cho, K. W., et al. (2011). Mol Cell Biol. 31(3):450-7.).

Western Blotting Analysis: A representative lot from an independent laboratory detected LCN13 in transfected mouse primary hepotcytes cell lysates (Sheng, L, et al. (2011). J Biol Chem. 286(44):38128-38135.).

Western Blotting Analysis: A representative lot detected LCN13 in mouse adipocyte cell lysates and in certain mouse organ tissues lysates (Zhou, Y. and Rui, L., (2013). Vitam Horm. 91:369-383.).

Quality

Evaluated by Western Blotting in mouse skin tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LCN13 in 10 µg of LCN13 mouse skin tissue lysate.

Target description

~20 kDa observed. Two observed isoforms at ~20 kDa and ~28 kDa are likely due to proteolytic cleavage of a common LCN13 precursor (Cho, K. W., et al. (2011). Mol Cell Biol. 31(3):450-7.). Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Mouse skin tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse. technique(s): western blot: suitable. NCBI accession no.: NP_705786. UniProt accession no.: Q8K1H9. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Obp2A(227627). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.

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