Anti-LGN Antibody (C15-1318-012)

General description

LGN is also known as G-protein-signaling modulator 2 (GPSM2). LGN has an important role in cellular structure and spindle pole orientation. LGN also stabilizes the apical complex during neuroblast divisions. LGN defects lead to Chudley-McCullough syndrome as well as a multiple micronuclei phenotype due to chromosomal mis-segregation and cell division errors.

Specificity

Other Homologies: Human (85% sequence homology), Chicken (75% sequence homology).

Immunogen

GST-tagged recombinant protein corresponding to mouse LGN.

Application

Anti-LGN Antibody is a highly specific rabbit polyclonal antibody, that targets LGN & has been tested in western blotting.

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LGN in mouse E16 brain tissue lysate.

Quality

Evaluated by Western Blotting in rat E16 heart tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LGN in rat E16 heart tissue lysate.

Target description

~77 kDa observed. Uncharacterized bands may be observed at ~30 kDa, ~60 kDa, and ~70 kDa in some cell lysates.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Heart tissue lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: mouse, rat. technique(s): western blot: suitable. NCBI accession no.: NP_083798. UniProt accession no.: Q8VDUO. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: mouse ... Gpsm2(76123). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.