General description
LIM Kinase 1 (LIMK1) is a protein kinase that plays an important role in cellular functions like cell motility and growth cone actin dynamics. LIMK1 contains one PDZ domain and two LIM domains which mediate protein to protein inteactions. It is widely expressed in the adult and fetal nervous system with the highest levels being found in the cerebral cortex. Mutations in LIMK1 have been associated with cardiovascular and musculo-skeletal abnormalities.
Specificity
The antibody recognizes LIMK1.
Immunogen
Epitope: Unknown
GST-tagged recombinant protein corresponding to a region of mouse LIMK1.
Application
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
This Anti-LIMK1 Antibody, clone 40/00-1B11-1-1 is validated for use in WB, IP, IC, IH for the detection of LIMK1.
Quality
Evaluated by Western Blot in PC3 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected LIMK1 on 10 µg of PC3 cell lysate.
Target description
~ 73 kDa
Physical form
Format: Purified
Protein G Purified
Purified in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
PC3 cell lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
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- UPC:
- 51202401
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB10750
- Temperature Control Device:
- Yes
