Anti-Lipoic Acid Rabbit pAb (C15-1303-565)

General description

Rabbit polyclonal antibody supplied as diluted serum. Recognizes native lipoic acid and lipoic acid covalently attached to proteins.

Recognizes native lipoic acid. Does not recognize lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products.

This Anti-Lipoic Acid Rabbit pAb is validated for use in ELISA, Frozen Sections, Immunoblotting, Paraffin Sections for the detection of Lipoic Acid.

Immunogen

lipoic acid conjugated to KLH

Application

ELISA (1:500-1:1000)

Frozen Sections (1:50-1:500)

Immunoblotting (1:1000-1:10,000)

Paraffin Sections (1:50-1:500)

Warning

Toxicity: Standard Handling (A)

Physical form

serum diluted in buffer, 50% glycerol, pH 7.5.

Other Notes

Does not bind lipoic acid modified by 4-HNE (4-hydroxy-2-noneal) and other lipid peroxidation products. Useful for assessing whether protein-bound lipoic acids have undergone certain forms of oxidative modification. The presence of protein-bound lipoic acid can be used as a marker for mitochondria. Variables associated with assay conditions will dictate the optimal working dilution.

Sasaki, M., et al. 2000. J. Autoimmun.15, 51.
Heitzer, T., et al. 2001. Free Radic. Biol. Med.31, 53.
Patrick. L. 2000. Altern. Med. Rev.5, 290.
Shi, S.S., et al. 1999. Antioxid. Redox Signal1, 123.
Humphries, K.M. and Szeda, L.I. 1998. Biochemistry37, 15835.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: rabbit. Quality Level: 100. antibody form: diluted serum. antibody product type: primary antibodies. clone: polyclonal. form: liquid. does not contain: preservative. species reactivity (predicted by homology): all. manufacturer/tradename: Calbiochem^®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. isotype: IgG. shipped in: wet ice. storage temp.: −. 70°C. target post-translational modification: unmodified. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.