General description

Rhombotin-2 (UniProt: P25791; also known as Cysteine-rich protein TTG-2, LIM domain only protein 2, LMO-2, T-cell translocation protein 2) is encoded by the LMO2 (also known as RBTN2, RBTNL1, RHOM2, TTG2) gene (Gene ID: 4005) in human. Rhombotin-2 is 158 amino acids, small cysteine-rich protein with multiple functions. A large portion of its structure is occupied by tandemly repeated cysteine-rich zinc-finger binding motifs, the LIM domains. However, it does not have any identifiable DNA-binding motifs. It is found predominantly in the nucleus or in the cytoplasm depending upon the tissue and the cell type. In kidney it is predominantly localized in the cytoplasm, but in hematopoietic cells it has nuclear localization. LMO2 is reported to be a proto-oncogene only in the context of T lymphocytes and is reported to play a role in T-cell leukemogenesis. It also has distinct functions in erythropoiesis. It acts with TAL1/SCL to regulate red blood cell development and interacts with LDB1 to maintain erythroid precursors in an immature state. It is reported to interact with retinoblastoma-binding protein 2 (RBP2) and human brain expressed X-linked (Bex2), both of which are shown to enhance its transcriptional activity. A chromosomal aberration involving LMO2 has been linked to T-cell acute lymphoblastic leukemia (T-ALL). (Ref.: Mao S., et al. (1997) Oncogene 14, 1531-1539).

Specificity

Clone 299B detects LMO-2/Rhombotin-2 in human tissues.

Immunogen

GST/His-tagged full length human recombinant LMO2/Rhombotin-2.

Application

Anti-LMO2 (Rhombotin-2), clone 299B Antibody, Cat. No. MABE1801, is a highly specific mouse monoclonal antibody that targets Rhombotin-2 and has been tested in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil, human placenta, and human lymphoma tissue.

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil tissues (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain).

Immunohistochemistry Analysis: A 1:10 dilution from a representative lot detected LMO2 (Rhombotin-2) in human tonsil and bone marrow tissues (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain).

Western Blotting Analysis: A representative lot detected LMO2 (Rhombotin-2) in OCILY19, SUDHL6, HEK/LMO2, and Ramos cell lysates (Courtesy of Giovanna Roncador, Ph.D., Centro Nacional de Investigaciones Oncologicas (CNIO), Madrid, Spain).

Immunocytochemistry Analysis: A representative lot detected LMO2 (Rhombotin-2) in HEK-V5-LMO2 transfected cells (Courtesy of Dr. Yasodha Natkunam, Department of Pathology, Stanford University School of Medicine, Stanford, CA, USA).

Research Category
Epigenetics & Nuclear Function

Quality

Evaluated by Immunohistochemistry in human bone marrow tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected LMO2 (Rhombotin-2) in human bone marrow tissue.

Target description

~18 kDa observed; 18.36 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibodyIgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.