ANTI-LNK/SH2B3 CLONE 13F1.2

General description

SH2B adapter protein 3 (UniProt Q9UQQ2; also known as Lymphocyte adapter protein, Lymphocyte-specific adapter protein Lnk, Signal transduction protein Lnk) is encoded by the SH2B3 (also known as IDDM, LNK) gene (Gene ID 10019) in human. LNK/SH2B3 is a SH2B family adaptor protein that functions as a negative regulator in hematopoiesis and cytokine signaling mediated by Janus kinase and receptor tyrosine kinases. Originally characterized in normal and malignant hematopoeitic cells, LNK is expressed in many other tissues, including kidney, brain, and various muscle types. Evidences indicate that LNK/SH2B3 also plays a negative regulatory role in TNF- and integrin signaling and actin cytoskeleton structure in both platelets and endothelial cells, affecting cell adhesion, migration, and thrombosis. Sh2b2-knockout in mice results in splenomegaly, abnormal lymphoid and myeloid homeostasis, increased hematopoietic progenitor cells and hypersensitivity to cytokines, while SH2B3 overexpression causes impaired expansion of lymphoid precursor cells and altered mature B-cell population. In addition, human SH2B3 gene has been linked to hypertension and renal disease by genome-wide association studies (GWASs), supporting the involvement of immune-mediated mechanisms in the pathogenesis of hypertension. Human LNK is a 575-a.a. protein that contains a dimerization domain (DD) and proline-rich regions in its N-terminal region, followed by a PH domain (a.a. 194-307) and SH2 domain (a.a. 364-462). The DD domain possesses a phenylalanine zipper motif that mediates the formation of homo- and heterodimers between SH2-B family members.

Specificity

Clone 13F1.2 targets an internal epitope conserved among human, mouse, and rat LNK/SH2B3.

Immunogen

GST-tagged recombinant human LNK/SH2B3 internal fragment.

Application

Detect LNK (SH2B3) using this mouse monoclonal Anti-LNK/SH2B3 Antibody, clone 13F1.2, Cat. No. MABS1178, validated for use in Western Blotting application.

Research Category
Signaling

Western Blotting Analysis: A 1:500 dilution from a representative lot detected LNK/SH2B3 in 10 µg of mouse RAW264.7 macrophage and rat L6 myoblast lysates.

Quality

Evaluated by Western Blotting in HUVEC lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected LNK/SH2B3 in 10 µg of HUVEC lysate.

Target description

~63 kDa observed. 63.23/60.49/60.39 kDa (human/mouse/rat) calculated. Note that the calculated molecular weight of rat protein is based on GenBank accession number AFP87272, but not any of the four sequences reported by UniProt P50745.

Physical form

Format: Purified

Protein G purified.

Purified mouse IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.