General description
LRFN4 is one of a family of five transmembrane glycoproteins that are highly expressed in neuronal tissues. LRFN proteins share leucine-rich repeat (LRR)-immunoglobulin-like (Ig)-fibronectin type III (Fn)-transmembrane domain structure with other members of the LRR-Ig-Fn protein superfamily such as the Slitrk family of proteins. Expression of LRFN1, -3, and -4 mRNA was detected in embryonic neuronal cells, while LRFN2 and LRFN5 expression was primarily restricted to more mature cells. LRFN1, -2, and -4 bound to PDZ domains of postsynaptic PSD95, re-distributing PSD95 to the cell periphery. It has been suggested that the LRFN proteins play a role in the developing and/or mature vertebrate nervous system.
Specificity
This antibody is predicted to not cross-react with other members of the LRFN family.
Immunogen
KLH-conjugated linear peptide corresponding to human LRFN4.
Application
Detect the LRFN4 protein using this Anti-LRFN4 validated for use in WB & IHC.
Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected LRFN4 in mouse brain tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
Quality
Evaluated by Western Blot in mouse brain tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected LRFN4 in 10 µg/mL of mouse brain tissue lysate.
Target description
~80 kDa observed. Uncharacterized bands may appear between ~40 and ~160 kDa in some lysates. The calculated molecular weight of this protein is 66 kDa, but can be observed at ~80 kDa due to high glycosylation.
Physical form
Antigen Affinity Purified
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Control
Mouse brain tissue lysate
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
biological source: rabbit. Quality Level: 300. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: mouse. species reactivity (predicted by homology): human (immunogen homology). technique(s): immunohistochemistry: suitable, western blot: suitable. UniProt accession no.: Q6PJG9. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... LRFN4(78999). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352203
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- ABN337