Anti-LTBP-1 Antibody (C15-1317-894)

General description

Latent-transforming growth factor beta-binding protein 1 (LTBP-1) is also called Transforming growth factor beta-1-binding protein 1 (TGF-beta1-BP-1). A large latent complex of TGFB1 from platelets is composed of the TGFB1 molecule non-covalently associated with a disulfide-bonded complex of a dimer of the N-terminal propeptide of the TGFB1 precursor and LTBP1. LTBP1 does not bind directly to active TGFB1 only to proforms. LTBP1 appears to be involved in the secretion of TGFB1 to sites at which it is stored or activated, and it is critical in controlling the action of TGFB1. LTBP1 may also play a role in extracellular matrix building and calcium binding mediated signaling surrounding TGFB1 activated signaling pathways. Also, because of its critical role in TGFB1 metabolism, LTBP1 is an important player in many developmental processes including embryo implantation and growth. LTBP1 is also a player in at least two distinct breast cancer metastasis signatures and has both a long and short isoform that work to establish and maintain the spatial restriction of TGFbeta bioavailability during development of many tissues including mammary development.

Immunogen

His-tagged recombinant protein corresponding to human LTBP-1.

Application

Immunocytochemistry Analysis: A representative lot detected LTBP-1 in transfected HEK293 cells (Chen, Q., et al. (2007). JBC. 282(36):26418-26430).
Immunocytochemistry Analysis: A representative lot detected LTBP-1 in rat FRC cells (Chen, Q., et al. (2007). JBC. 282(36):26418-26430).

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

This Anti-LTBP-1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of LTBP-1.

Quality

Evaluated by Western Blotting in human lung tissue lysate using a non-reduced gel.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LTBP-1 in 10 µg of human lung tissue lysate using a non-reduced gel.

Target description

~220 kDa observed. Uncharacterized band(s) may appear in some lysates.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: serum. antibody product type: primary antibodies. clone: polyclonal. species reactivity: human, rat. technique(s): immunocytochemistry: suitable, western blot: suitable. NCBI accession no.: NP_000618. UniProt accession no.: Q14766. shipped in: dry ice. target post-translational modification: unmodified. Gene Information: human ... LTBP1(4052). Storage Class Code: 10 - Combustible liquids. WGK: WGK 1.