ANTI-LYSINE-SPECIFIC DEMETHYLASE 5C ANTIBODY (C15-1317-185)

General description

Lysine-specific demethylase 5C is also called Histone demethylase JARID1C, Jumonji/ARID domain-containing protein 1C, Protein SmcX and Protein Xe169. Lysine-specific demethylase 5C is a histone demethylase that specifically targets trimethylated and dimethylated, but not monomethylated, ′Lys-4′ of histone H3, making it a regulator of the histone code. Lysine-specific demethylase 5C is involved in transcriptional repression of neuronal genes by recruiting histone deacetylases and REST at neuron-restrictive silencer elements and is associated with Mental retardation, X-linked, syndromic, Claes-Jensen type (MRXSCJ). Lysine-specific demethylase 5C is widely expressed with highest levels found in brain and skeletal muscle.

Specificity

This antibody is expected to react with all four known isoforms based upon immunogen design.

Immunogen

KLH-conjugated linear peptide corresponding to a region near the near the N-terminus of human Lysine-specific demethylase 5C.

Application

Detect JARID using this rabbit polyclonal antibody, Anti-Lysine-specific demethylase 5C Antibody validated for use in western blotting, IP & IHC.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Lysine-specific demethylase 5C in human stomach tissue.

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Histones

Quality

Evaluated by Western Blotting in human hippocampus tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Lysine-specific demethylase 5C in 10 µg of human hippocampus tissue lysate.

Target description

~170 kDa observed. Uniprot describes four isoforms at 176 kDa (isoform 1), 175 kDa (isoform 2), 171 kDa (isoform 3), and 155 kDa (isoform 4). Uncharacterized band(s) may be observed in some cell lysates.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

biological source: rabbit. Quality Level: 100. antibody form: affinity isolated antibody. antibody product type: primary antibodies. clone: polyclonal. purified by: affinity chromatography. species reactivity: human. technique(s): immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable. NCBI accession no.: NP_004178.2. UniProt accession no.: P41229. shipped in: wet ice. target post-translational modification: unmodified. Gene Information: human ... KDM5C(8242). Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.