Specificity
Shows selective staining of dendritic trees throughout the brain in immunohistochemical staining of brain tissue. In perkinje neurons, staining is restricted to the dendrites distal to the initial dendrite segment. No staining of cell body in mature Perkinje cells but strong staining of cell bodies of developing Perkinje cells from neonatal rats. No detectable staining of axons or non-neuronal cells. Reacts specifically with all forms of MAP2 (MAP2a ~280 kDa, MAP2b ~280 kDa and MAP2c ~70 kDa) as determined by immunoblot. No cross-reactivity observed to other MAPs or tubulin.
Immunogen
Rat brain microtubule-associated protein.
Application
Anti-MAP2A Antibody, 2B, 2C, clone HM-2 detects level of MAP2A & has been published & validated for use in WB, IH.
Immunoblotting at 1:500 using fresh rat brain extract or enriched microtubule protein preparation.
Immunohistochemistry: 1:50 to 1:1000
Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Physical form
Ascites fluid. Liquid with 15 mM sodium azide.
Storage and Stability
Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Shipping Information:
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- UPC:
- 41181549
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- MAB364
- Temperature Control Device:
- Yes
